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Endoplasmic reticulum

The Endoplasmic reticulum fluxes include ryanodine receptor (RyR) flux (Jrel), SERCA flux (Jup), SR leakage (Jleak), and calcium diffusion from NSR to JSR (Jtr).

JCaSR=VNSRVsubSR(JleakJup)+JrelJrel=kRyRPO1RyR(caJSRcasr)Jtr=ktrCaSR(caJSRcaNSR)Jleak=0.5(1+5RyRCKp)kSRleakJup=VmaxSRfSRrSR1+fSR+rSRddtPO1RyR=kaposRyRH(casr,KmRyR,4)PC1RyRkanegRyRPO1RyRddtcaJSR=βSR(JrelVsubSR+JtrVNSR)/VJSRddtcaNSR=JupJleakJtrβSR=(caJSR+Kmcsqn)2(caJSR+Kmcsqn)2+ΣCsqnKmcsqnfSR=(casrKmfp)2rSR=(caNSRKmrSR)2KmRyR=3.51expit(caJSR530200)+0.25PC1RyR=1PO1RyRKmfp=min(fCKIIPLB,fPKAPLB)fPKAPLB=(10.5531)1fracPLBpfracPKAPLBo+0.5531fCKIIPLB=(10.5fracPLBCKp)fracPLBCKp=0.2CaMKAct\begin{align} J_{CaSR} &= \frac{V_{NSR}}{V_{subSR}} (J_{leak} - J_{up}) + J_{rel} \\ J_{rel} &= k_{RyR} \cdot PO1_{RyR} \cdot (ca_{JSR} - ca_{sr}) \\ J_{tr} &= ktrCa_{SR} (ca_{JSR} - ca_{NSR}) \\ J_{leak} &= 0.5 (1 + 5 RyR_{CKp}) kSR_{leak} \\ J_{up} &= Vmax_{SR} \frac{fSR - rSR}{1 + fSR + rSR} \\ \frac{d}{dt} PO1_{RyR} &= kapos_{RyR} \cdot H(ca_{sr}, Km_{RyR}, 4) \cdot PC1_{RyR} - kaneg_{RyR} \cdot PO1_{RyR} \\ \frac{d}{dt} ca_{JSR} &= \beta_{SR} (-J_{rel} V_{subSR} + J_{tr} V_{NSR}) / V_{JSR} \\ \frac{d}{dt} ca_{NSR} &= J_{up} - J_{leak} - J_{tr} \\ \beta_{SR} &= \frac{(ca_{JSR} + Km_{csqn})^2}{(ca_{JSR} + Km_{csqn})^2 + \Sigma Csqn Km_{csqn}} \\ fSR &= \left( \frac{ca_{sr}}{Kmfp} \right)^2 \\ rSR &= \left( \frac{ca_{NSR}}{Kmr_{SR}} \right)^2 \\ KmRyR &= 3.51 \cdot expit(-\frac{ca_{JSR} - 530}{200}) + 0.25 \\ PC1_{RyR} &= 1 - PO1_{RyR} \\ Kmfp &= \min(fCKII_{PLB}, fPKA_{PLB}) \\ fPKA_{PLB} &= (1 - 0.5531) \frac{1 - fracPLBp}{fracPKA_PLBo} + 0.5531 \\ fCKII_{PLB} &= (1 - 0.5 * fracPLB_{CKp}) \\ fracPLB_{CKp} &= 0.2CaMKAct \\ \end{align}